Top
hERG
Recombinant Cell Line:
CHO-K1
and HEK293 cells are transfected to stably overexpress
functional hERG (Ether-a go-go Related Gene). Cells
are functional for use in patch-clamp assays.
Our cell lines have been functionally
validated using electrophysiological techniques. Predicted
sensitivity to known pharmacological agents (Dofetilide,
Cisapride, and Terfenadine) has been demonstrated. All
pharmacological values are comparable with data previously
reported in peer-reviewed journals.
Top
Action
Potential Duration Assays (APD)
Action
potential recordings reflect the integrated activities
of the multiple ion channels, pumps, and exchanger mechanisms
that contribute to the depolarization phase and repolarization
process at the cellular level. Therefore, assessing
changes in action potential duration can be a comprehensive
approach for identifying effects of a test substance
on repolarization. When a positive effect on one of
the action potential parameters is observed in an in
vitro assay, ionic current studies should be conducted
to elucidate the cellular mechanism for the effect.
Mammalian
cardiac action potentials in vitro:
[RMP,
APA, APD60 and APD90 ]
Other
primary cardiac ion channels :
[Na,
Ca, KvLQT1/minK & Kv4.3]
Top
Other
Services
Avan
Biotech has a group of scientists with solid background,
recognized expertise and broad knowledge about physiology
of ion channel/receptors and channelopathies including
those in the central nervous system (CNS). Avan Biotech
offers services on Screening or Assay development regarding
any channels or receptors.
If you are interested in discussing protocol development
or any other cardiac safety issues, you can contact
our principal scientists.
Top
|
